Support the 3Rs (Replacement, Reduction and Refinement) through the use of organoids

This Summer School is organized in the framework of EuroFAANG, a European Infrastructure project (2023 –2025) that will develop a concept for a sustainable pan-European infrastructure to facilitate genotype-to-phenotype (G2P) research across various farmed animal species.

The summer school will gather speakers from different European countries to propose a state-of-the-art overview of research on organoids/organ-on-chips, with a specific focus on farm animal species. Note that we will not deal with cancer.

Sessions will cover a large field of organs, functions, with applications in nutrition, health and reproductive biotechnologies. A virtual demonstration on the making of organoids will be proposed.

A round table will be organized with different speakers representative of these different fields as well as a representative of an animal feeding company.

Groups of participants (4-5 per group) will be formed at the beginning of the Summer school, based on research and species of interest. Each group will choose a subject in relation to their specific interest. They will present the result of their work at the end of the summer school.

FOR WHOM ?

PhD students, post-docs, engineers, early career researchers (EU residents).

HOW TO APPLY

Please send your application including a Curriculum Vitae and a letter of intent explaining why you would like to attend this summer school and your scientific project (species and organs of interest) to: summerschool.eurofaang@inrae.fr.

Deadline: June 20*, 2023.  *The application deadline was extended until June 27, 2023.

COSTS : The summer school is free of charge. Lunch will be provided to participants. 

Place: building 400 (ERIST) large room, INRAe, Jouy-en-Josas 78350

PROGRAM

Monday, September 11 

13:30 – 17:30

• Elisabetta Giuffra (INRAE, France): Presentation of EuroFAANG objectives.
• Alice Jouneau (INRAE, France): Presentation of the week, groups and tasks.

1^st session: Overviews

• Bertrand Pain (SBRI, INRAE, France): Embryonic and Adult stem cells: introduction to organoids.
• Peter Harrison (EBI, UK): Improving cellular model metadata descriptions for quality control, reproducibility and accelerating science.
• Esther Ellen (WUR, Netherlands): Organoids in animal breeding and nutrition research: opportunities and challenges.

Tuesday, September 12

9:30 – 11:00

2^nd session: Tools and methodologies

• Giorgia Egidy-Maskos (GABI, INRAE, France): Transgenesis in organoids.
• Severine Le Gac (UTwente, Netherlands): Organ-on-a-chip platforms for biological and medical applications.
• Matthieu Simion (BREED, INRAe, France): Organoid imaging using light sheet microscopy.

11:00 – 11:30 : Coffee break

11:30 – 12:30

3^rd session: Gut organoids and their applications

• Maxime Mahé, (Inserm, France): Modelling intestinal development and physiology using human stem cell-derived organoid.
• Fulvio Gandolfi (University of Milan, Italy): 3D organotypic intestinal cultures of fish intestine for evaluating sustainable feeds.

12:30 – 14:00 : Lunch buffet on site

14:00-14:30 : 3^rd session: Gut organoids and their applications (continued)

• Martin Beaumont (GENPHYSE, INRAE, France): Intestinal organoids: innovative in vitro tools to study host-microbiota interactions in farm animals.

14:30 – 17:00

4^th session: Organoids to study host-pathogen interactions in gut and lung

• Kate Sutton (University of Edimburgh, UK): Apical-out chicken enteroids with lamina propria leukocytes as a model to study host-pathogen interactions.
• Fabienne Archer (IVPC, INRAE, France): Lung organoids and air-liquid interface cultures to better understand host-pathogen interactions in mammals.
• Maud Contrant (Anses, France): Contribution of organoids to the study of host-pathogen interactions.

Wednesday, September 13

9:30 – 12:30

5^th session: Reproduction and Development

• Ramiro Alberio (Nottingham university, UK): Modelling gastrulation in vitro using pluripotent cells.
• Marcia Ferraz (LMU, Germany): Use of decellularized extracellular matrix hydrogels to create complex in vitro reproductive models.

10:30 – 11:00 : Coffee break

• Bart Gadella (Utrecht University, Netherlands): An oviduct-on- a-chip culture system for animal embryo production ex vivo.
• Karine Reynaud (PRC, INRAE, France): Oviduct spheroids and organoids: new in vitromodels to better understand fertility.
• Tiziana Brevini (University of Milan, Italy): Generation of decellularized scaffolds to study the role of ovarian extracellular matrix.

12:30 – 14:00 : Lunch buffet on site

14:00 – 15:00

6^th session: Issues raised by in vitro cellular models for biobanking

Michelle Tixier-Boichard and Temitope Oluwafisayo Ige (GABI, INRAE, France): Presentation followed by general discussion after the results of the first EuroFAANG survey.

15:00 – 17:00 Virtual demonstration

Virtual demonstration of intestinal organoid generation and characterization – Stem Cell Technologies.

Thursday, September 14  

9:30 – 10:30

7^th session: Ethics 

• Franck Meijboom (Utrecht University, Netherlands): Answering and raising ethical questions at the same time: on the ethics, 3R principles and using organoids.
• Athanassia Sotiropoulos (FC3R, France): What is FC3R?

10:30 – 11:00 : Coffee break

11:00 – 12:30 : Round table: Properties associated with the use of organoids to study physiology and phenotype of a whole animal.

12:30 – 14:00 : Lunch buffet on site

Thursday afternoon

Work in small groups to prepare next day's presentation.

Friday, September 15  

9:00 – 16:00

Morning

Presentation of the work of each group.

Afternoon

Survey filling and wrapping up.